COOMASSIE^nano Protein Staining Solution

COOMASSIE^nano, enhanced by nano-technology, is a ready-to-use protein staining solution for SDS-PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, COOMASSIEnano is considered to be safe and environmentally friendly. COOMASSIEnano is also compatible with mass spectrophotometry.

1. Carefully shift COOMASSIE^nano up and down few times before use.
2. After SDS-PAGE has finished, remove the gel from the cassette and submerge it in a proper amount of COOMASSIE^nano dye, enough to cover the gel. Lightly agitate the staining box for 10 to 30 minutes at room temperature.
3. As there is no need for washing or destaining, please continue to carefully remove the dye solution and rinse the excess dye in the gel with water.
4. Image the gel.

COOMASSIE^nano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.

COOMASSIEnano Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis. After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIEnano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.

Important notes

• Container: box for gel staining
• Shaker: orbital or rocking shaker